The Presto™ Mini gDNA Yeast Kit offers a simple and gentle reagent DNA precipitation method for isolating high molecular weight genomic, mitochondrial or viral DNA from Saccharomyces cerevisiae and a variety of other yeast and fungus species. This highly versatile solution based system offers a convenient procedure with minimal hands on time. The provided Sorbitol Buffer, when combined with ?-mercaptoethanol, zymolase or lyticase, will efficiently lyse yeast and other fungus species cell walls consisting of chitin and polysaccharides. Purified DNA (A260/A280 = 1.8-2.0), is suitable for use in PCR or other enzymatic reactions.
Purify genomic DNA from (up to 2 x 108) yeast and other fungus species using a simple and gentle 3 buffer system
Simple and gentle reagent DNA precipitation method
Convenient premixed Sorbitol Buffer!
Elution volume: 50-100 µl
Time: 40 minutes
Storage: dry at room temperature (15-25°C) for up to 1 year
Applications
DNA archiving, PCR, AFLP, RFLP/PADP, Southern Blotting, Real-time PCR
Components
Sorbitol Buffer
Cell Lysis Buffer
Protein Removal Buffer
DNA Hydration Buffer
Quality Control
The quality of the Presto™ Mini gDNA Yeast Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. The purified genomic DNA (A260/A280 ratio of 1.8-2.0) is quantified with a spectrophotometer and checked by electrophoresis.
Codice Prodotto: GBY100
