The Presto™ Mini gDNA Bacteria Kit is a Bacteria genomic DNA extraction kit optimized for genomic and viral DNA purification from Gram negative bacterial cells and Gram positive bacterial cells. This Bacteria Genomic DNA Extraction Kit includes Gram+ Buffer and Lysozyme and when combined, will efficiently lyse bacterial cell walls consisting of the peptidoglycan layer. Chaotropic salt is used to lyse cells and degrade protein, allowing DNA to easily bind to the glass fiber matrix of the genomic DNA spin column. Contaminants are removed using a Wash Buffer (containing ethanol) and the purified genomic DNA is eluted by a low salt Elution Buffer, TE or water. The entire procedure can be completed within 30 minutes without phenol/chloroform extraction or alcohol precipitation. The purified DNA, with approximately 20-40 kb, is suitable for use in PCR or other enzymatic reactions.
Protocol download
Advantages (Cat. # GBB100, GBB101, GBB300, GBB301)
Purify genomic DNA within 30 minutes!
High Yield: up to 40 ?g (1 x 109 Escherichia coli) and 15 µg (1 x 109 Bacillus subtilis)
High Purity gDNA: A260/A280 >1.9
DNA Size: 20-40 kb
Samples: Gram (+) Positive and Gram (-) Negative bacterial cells (up to 1 x 109), 200 µl of blood and biological fluids such as plasma and urine
Convenient: Includes Gram+ Buffer for preparing Lysozyme solutions to speed up sample preparation when purifying DNA from Gram (+) positive bacteria
Genomic DNA glass fiber spin column (green membrane optimized for genomic DNA purification from bacteria)
Elution volume: 30-200 µl
Storage: dry at room temperature (15-25°C) for up to 1 year
Applications
PCR, Restriction Endonuclease Digestions, Array, Southern Blots and various other enzymatic reactions
Components
Proteinase K
Gram+ Buffer
GT Buffer
GB Buffer
W1 Buffer
Wash Buffer
Lysozyme
Elution Buffer (10 mM Tris-HCl, pH8.5 at 25°C)
GD Columns
2 ml Collection Tubes
Quality Control
The quality of the Presto™ Mini gDNA Bacteria Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. The purified genomic DNA from Escherichia coli cells (up to 1 x 109), (up to 40 µg with an A260/A280 ratio of >2.0) is quantified with a spectrophotometer and analyzed by electrophoresis.
Codice Prodotto: GBB100
