The Prestained Protein Ladder V is a three-color prestained protein standard which includes protein dye for convenient protein sample staining. The protein ladder consists of 10 prestained proteins covering a wide range of molecular weights (10 to 180 kDa). Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band) when separated on SDS-PAGE. The Ladder is designed for monitoring protein separated during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximate sizing of proteins. The ladder is supplied in a gel loading buffer and is ready to use, without heating, diluting, or a reducing agent prior to loading.
Advantages:
Wide Molecular Weight range: 10-180 kDa
Supplied buffer for direct gel loading
Sharp bands with 28 and 75 KDa reference bands (green/red dye)
Includes Protein Loading Dye (2 ml, 5X)
3 ?l or 5 ?l per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively
2~3 ?l per well for general Western transferring
Increase application amount for thicker or larger gel
Optional Dithiothreitol (DTT) procedure
Storage:
25ºC for 2 weeks
4ºC for 3 months
-20ºC for 24 months
Applications:
Monitor protein migration/sample in SDS-PAGE, Monitor protein transfer onto membranes during Western Blotting, Sizing of proteins on SDS-PAGE and Western blots
Components:
Prestained Protein Ladder V (approximately 0.2~0.4 mg/ml of each protein in buffer {20 mM Trisphosphate pH7.5 at 25°C, 2% SDS, 1 mM 2-Mercaptoethanol, 3.6 M Urea and 15% (v/v) glycerol}.
Protein Loading Dye (2 ml, 5X)
Quality Control:
The quality of the Prestained Protein Ladder V is tested on a lot-to-lot basis according to Geneaid's ISO certified quality management system. 5 ?l of Prestained Protein Ladder V resolves 10 bands in 4-20% SDS-PAGE (Tris-glycine buffer) and after Western blotting to PVDF membrane.
Codice Prodotto: PL005
