The Genomic DNA Mini Kit (Blood/Cultured Cell) is a genomic DNA extraction kit optimized for genomic, mitochondrial and virus DNA purification from whole blood, buffy coat and cultured cell samples. This Genomic DNA Extraction Kit uses RBC Lysis Buffer and chaotropic salt to lyse cells and degrade protein, allowing DNA to bind to the glass fiber matrix of the genomic DNA spin column. Contaminants are removed using a Wash Buffer (containing ethanol) and the purified genomic DNA is eluted by a low salt Elution Buffer, TE or water. Phenol/chloroform extraction or alcohol precipitation is not required and the purified genomic DNA (approximately 20-30 kb) is suitable for use in PCR or various other enzymatic reactions. For RNA purification from blood and cultured cell samples, the Total RNA Mini Kit (Blood/CC) is also available.
Advantages:
Purify genomic DNA within 25 minutes!
High Yield, High Quality Genomic DNA: 4-6 ?g of pure genomic DNA (A260/A280 = 1.8-2.0) from 200 µl of whole blood
Variety of Samples: up to 300 µl of whole blood, up to 200 µl of buffy coat, up to 1 x 107 cultured cells, up to 2 x 108 yeast and other fungus species
Genomic DNA glass fiber spin columns (green membrane optimized for genomic DNA extraction)
Storage: dry at room temperature (15-25ºC) for up to 1 year
Applications
PCR, AFLP/PADP, RFLP, Southern Blotting, Real-time PCR
Components
RBC Lysis Buffer
GT Buffer
GB Buffer
W1 Buffer
Wash Buffer
Elution Buffer (10 mM Tris-HCl, pH8.5 at 25ºC)
GD Columns
2 ml Collection Tubes
Quality Control
The quality of the Genomic DNA Mini Kit (Blood/Cultured Cell) is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. Genomic DNA from 200 ?l of whole human blood is extracted using the Genomic DNA Mini Kit (Blood/Cultured Cell). The purified DNA (5 ?g with an A260/A280 ratio of 1.8-2.0) is quantified with a spectrophotometer and checked by electrophoresis.
Codice Prodotto: GB100
