The Magnetic Beads Genomic DNA Extraction Kit Bacteria was designed specifically for efficient genomic DNA purification from Gram (-) negative and Gram (+) positve bacteria. DNA is bound to the surface of the magnetic beads and released using a proprietary buffer system. The kit can be easily adapted to automated magnetic bead separation instruments and workstations. The purified DNA can be used in qPCR and a variety of other downstream applications.
Advantages:
High Yield: >26 µg of Genomic DNA
High Quality DNA: A260/A280 = 1.8-2.0
Easily adapted to automated magnetic bead separation instruments and workstations
Sample: 2 x 109 of bacteria cells (Gram + and Gram -)
Manual or automated DNA isolation
Operation time: within 30 minutes (manual)
Storage: dry at room temperature (15-25ºC) for up to 1 year, Proteinase K (4ºC), Lysozyme (-20ºC)
Applications:
qPCR, PCR, Real-time PCR, Automated Fluorescent DNA Sequencing, Southern Blotting, Next Generation Sequencing (NGS)
Components:
ME1 Buffer
ME2 Buffer
ME3 Buffer
ME4 Buffer
Lysozyme
Proteinase K
MW1 Buffer
MW2 Buffer
ME Magnetic Beads
Elution Buffer
Quality Control:
The quality of the The Magnetic Beads Genomic DNA Extraction Kit Bacteria is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system by isolating genomic DNA from 1 x 109 cells of Escherichia coli and from 1 x 109 cells of Bacillus subtilis. 5 ?l of extracted DNA product from a 100 ?l eluate was analyzed on a 0.8% agarose gel.
Codice Prodotto: ME096
