Advantages
Recombinant sourced Taq DNA Polymerase
Format: 500 U and 2,500 U
Available with or without dNTPs
Amplification: up to 8 kb
Storage: -20ºC for extended periods
Shipping: 2-8ºC (dry ice is not required)
3' to 5' Exonuclease Proofreading Ability: NO
5' to 3' Exonuclease Activity: YES
Applications
Screening, Primer Extension, Amplification, Terminal dA Tailing
Source
Taq DNA Polymease is expressed and purified from an E. coli strain which carries the Taq DNA Polymerase gene from Thermus aquaticus.
Composition
Storage Buffer: 20 mM Tris-HCl, ph8.0, 0.1 EDTA, 1 mM DTT, 1.0% Triton X-100, 50% Glycerol.
10X PCR Buffer: 150 mM Tris-HCl, pH8.75 at 25ºC, 500 mM KCl, 20 mM MgCl2, 1.0% Triton X-100. The following compositions are available alternatives:
MgCl2 separate
Pre-mixed with MgSO4
All Taq DNA Polymerase compositions are available with or without dNTPs. Please see the order information below to determine which configuration best suits your requirement.
Unit Definition
One unit incorporates 10 nmol of dNTP into acid insoluble material in 30 minutes at 74ºC.
Quality Control
The quality of Taq DNA Polymerase is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. Quality control assays include activity test, PCR, endonuclease activity, exonuclease activity.
Codice Prodotto: TQN50
